详细描述:
人ESR1探针包括一组Quasar® 570标记的寡核苷酸以一定的比例混合在一起,终浓度是5 nmol,按照标准条件操作能够做200-400次杂交。该探针用于利用荧光原位杂交的方法检测人ESR1的转录本。
探针设计依据:Product was designed against estrogen receptor 1 ESR1 a.k.a. ER; ESR; Era; ESRA; ESTRR; NR3A1 (NCBI gene ID:2099) and nucleotides 687-877, 878-994, 995-1330, 1331-1469, 1470-1603 of NM_000125.3. This is an inclusive and exclusive probe set designed to also detect the following variants: NM_001122740, NM_001122741, NM_001291230, XM_006715374, XM_006715375, ENST00000338799, ENST00000427531, and ENST00000440973, and partially: ENST00000206249 (exons 14 and 15a), ENST00000406599, (exon 15a), ENST00000415488 (exons 10, 14, 15b), ENST00000443427 (exons 9, 10, 14, 15a), ENST00000456483 (exons 9, 10, 14, 15a), and ENST00000482101 (exon 11), but NOT: ENST00000404742, ENST00000446550, ENST00000473497, or ENST00000488573. Note: Avoids similar sequence to ESR2, ESRRA, ESRRB, and ESRRG within second common exon.
该探针没有在同物种或其它物种的同源和直系同源序列上验证可能的交叉杂交。
属性:
更大激发荧光(Lambda Max):548;
更大发射荧光:566;
产品用法:
溶解后的探针注意尽量避免反复冻融。当天或短期使用,探针溶液可以避光放在+2 ~ +8 °C储存一个月,超过1个月,推荐分装后将溶解后的探针放在-15至-30 °C避光保存。
Protocols:详细Protocol根据样本类型而异,请上以下网站下载:https://www.biosearchtech.com/stellarisprotocols;
储存及运输:
运输条件:常温/蓝冰/干冰;
储存条件:+2 ~ +8 °C;
Properties:
Product Usage:
Absorption Maximum (Lambda Max): 647;
Fluorescence Maximum: 670;
Product Usage:
Please note that reconstituted probe mix should be subjected to a minimum number of freeze-thaw cycles. For daily and short-term use, the mix can be stored at +2 to +8 °C in the dark for up to a month. For long-term use: We recommend freezing reconstituted probes in the dark at -15 to -30 °C for storage lasting longer than a month. Protocols: Detailed protocols by sample type can be found here: https://www.biosearchtech.com/stellarisprotocols.
Storage and Handling:
Shipping Conditions: Ambient;
Storage Conditions: +2 to +8 °C