详细描述：
人ESR1探针包括一组CAL Fluor® Red 590标记的寡核苷酸以一定的比例混合在一起，终浓度是5 nmol，按照标准条件操作能够做200-400次杂交。该探针用于利用荧光原位杂交的方法检测人ESR1的转录本。
探针设计依据：Product was designed against estrogen receptor 1 ESR1 a.k.a. ER; ESR; Era; ESRA; ESTRR; NR3A1 (NCBI gene ID:2099) and nucleotides 687-877, 878-994, 995-1330, 1331-1469, 1470-1603 of NM_000125.3. This is an inclusive and exclusive probe set designed to also detect the following variants: NM_001122740, NM_001122741, NM_001291230, XM_006715374, XM_006715375, ENST00000338799, ENST00000427531, and ENST00000440973, and partially: ENST00000206249 (exons 14 and 15a), ENST00000406599, (exon 15a), ENST00000415488 (exons 10, 14, 15b), ENST00000443427 (exons 9, 10, 14, 15a), ENST00000456483 (exons 9, 10, 14, 15a), and ENST00000482101 (exon 11), but NOT: ENST00000404742, ENST00000446550, ENST00000473497, or ENST00000488573. Note: Avoids similar sequence to ESR2, ESRRA, ESRRB, and ESRRG within second common exon.
该探针没有在同物种或其它物种的同源和直系同源序列上验证可能的交叉杂交。
Stellaris® FISH Probes, Human ESR1 with CAL Fluor® Red 590 Dye consists of a set of dye-labeled oligos mixed and pooled into a final delivered amount of 5 nmol, which yields approximately 200-400 hybridizations under standard conditions. Designed to detect ESR1 transcripts in specimens using fluorescence in situ hybridization (FISH). Design Criteria: Product was designed against estrogen receptor 1 ESR1 a.k.a. ER; ESR; Era; ESRA; ESTRR; NR3A1 (NCBI gene ID:2099) and nucleotides 687-877, 878-994, 995-1330, 1331-1469, 1470-1603 of NM_000125.3. This is an inclusive and exclusive probe set designed to also detect the following variants: NM_001122740, NM_001122741, NM_001291230, XM_006715374, XM_006715375, ENST00000338799, ENST00000427531, and ENST00000440973, and partially: ENST00000206249 (exons 14 and 15a), ENST00000406599, (exon 15a), ENST00000415488 (exons 10, 14, 15b), ENST00000443427 (exons 9, 10, 14, 15a), ENST00000456483 (exons 9, 10, 14, 15a), and ENST00000482101 (exon 11), but NOT: ENST00000404742, ENST00000446550, ENST00000473497, or ENST00000488573. Note: Avoids similar sequence to ESR2, ESRRA, ESRRB, and ESRRG within second common exon. The probe set has not been tested for potential cross-hybridization to RNA(s) of paralogous and orthologous gene(s) in the same or other species.
