详细描述:
GFP探针包括一组CAL Fluor® Red 590标记的寡核苷酸以一定的比例混合在一起,终浓度是5 nmol,按照标准条件操作能够做200-400次杂交。该探针用于利用荧光原位杂交的方法检测GFP的转录本。
探针设计依据:Product was designed against Green Fluorescent Protein GFP a.k.a. wtGFP, GFPs65t (NCBI gene ID:) and nucleotides 1110-1823 of GQ334691.1. This is an inclusive probe set designed to also detect the following variants:>GFP (WT) Clontech 632370, >GFP (WTs65c) based on 632370, >GFP(WTuv)Clontech 632312, >X83959_GFP1 gi|634008|emb|X83959.1|, >X83960_GFP2 gi|634010|emb|X83960.1|, >L29345_GFP gi|606383|gb|L29345.1|, >M62653_GFP gi|155660|gb|M62653.1|, >Tsien_EGFP1 gi|2081984|gb|I41394.1|I41394, >AF324407_AZOVI_GFP gi|26892272|gb|AF324407.1|, >U73901_GFP3 gi|1658074|gb|U73901.1|AVU73901.
该探针没有在同物种或其它物种的同源和直系同源序列上验证可能的交叉杂交。
Stellaris® FISH Probes, GFP with CAL Fluor® Red 590 Dye consists of a set of dye-labeled oligos mixed and pooled into a final delivered amount of 5 nmol, which yields approximately 200-400 hybridizations under standard conditions. Designed to detect GFP transcripts in specimens using fluorescence in situ hybridization (FISH). Design Criteria: Product was designed against Green Fluorescent Protein GFP a.k.a. wtGFP, GFPs65t (NCBI gene ID:) and nucleotides 1110-1823 of GQ334691.1. This is an inclusive probe set designed to also detect the following variants:>GFP (WT) Clontech 632370, >GFP (WTs65c) based on 632370, >GFP(WTuv)Clontech 632312, >X83959_GFP1 gi|634008|emb|X83959.1|, >X83960_GFP2 gi|634010|emb|X83960.1|, >L29345_GFP gi|606383|gb|L29345.1|, >M62653_GFP gi|155660|gb|M62653.1|, >Tsien_EGFP1 gi|2081984|gb|I41394.1|I41394, >AF324407_AZOVI_GFP gi|26892272|gb|AF324407.1|, >U73901_GFP3 gi|1658074|gb|U73901.1|AVU73901. The probe set has not been tested for potential cross-hybridization to RNA(s) of paralogous and orthologous gene(s) in the same or other species.
属性:
更大激发荧光(Lambda Max):569;
更大发射荧光:591;
产品用法:
溶解后的探针注意尽量避免反复冻融。当天或短期使用,探针溶液可以避光放在+2 ~ +8 °C储存一个月,超过1个月,推荐分装后将溶解后的探针放在-15至-30 °C避光保存。
Protocols:详细Protocol根据样本类型而异,请上以下网站下载:https://www.biosearchtech.com/stellarisprotocols;
储存及运输:
运输条件:常温/蓝冰/干冰;
储存条件:+2 ~ +8 °C;
Properties:
Product Usage:
Absorption Maximum (Lambda Max): 647;
Fluorescence Maximum: 670;
Product Usage:
Please note that reconstituted probe mix should be subjected to a minimum number of freeze-thaw cycles. For daily and short-term use, the mix can be stored at +2 to +8 °C in the dark for up to a month. For long-term use: We recommend freezing reconstituted probes in the dark at -15 to -30 °C for storage lasting longer than a month. Protocols: Detailed protocols by sample type can be found here: https://www.biosearchtech.com/stellarisprotocols.
Storage and Handling:
Shipping Conditions: Ambient;
Storage Conditions: +2 to +8 °C