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NP-Fluorescein-AECM-FICOLL

NP-Fluorescein-AECM-FICOLL

产品概述 技术指标 配套产品 订购信息 精彩视频 相关资料
详细描述:
荧光素与NP-氨基乙基羧基甲基-聚蔗糖(高分子量多糖)偶联。 Hapten-FICOLL偶联物可用作研究B淋巴细胞活化的T非依赖性抗原。 Fluorescein is conjugated to NP-AminoEthylCarboxyMethyl-FICOLL, a high molecular weight polysaccharide. Hapten-FICOLL conjugates are useful as T-independent antigens for studying B lymphocyte activation.
Properties: Chemical Name: 4-Hydroxy-3-nitrophenylacetic -AminoEthylCarboxyMethyl-FICOLL-Fluorescein; Appearance: orange powder; Absorption Maximum (Lambda Max): 430; Extinction Coefficient at Lambda max: 4230; Product Usage: This product can be dissolved in PBS buffer, water or other neutral buffers at a concentration of 1mg/mL. Product may precipitate when stored in solution, therefore it is recommended to centrifuge the solutions briefly before use and use only the supernatant. Store solution in aliquots at -20°C. Protect from light. Conjugation is determined at pH 8.5 by measuring NP absorbance at 430 nm, using 4.23 x 10E+03 as the extinction coefficient for NP, and is given as a molar ratio of NP to Ficoll assuming the molecular weight of Ficoll is 400,000. Conjugation for Fluorescein, is determined by measuring Fluorescein absorbance at 495 nm, using 8.3 x 10E+04 as the extinction coefficient for Fluorescein, and is given as a molar ratio of Fluorescein to Ficoll assuming the molecular weight of Ficoll is 400,000. Storage and Handling: Shipping Conditions: Ambient; Storage Conditions: Room Temperature
货号 产品名称 品牌 购买
货号 名称 单位 购买
F-1420F-1 NP-Fluorescein-AECM-FICOLL 1 mg 商城订购
F-1420F-10 NP-Fluorescein-AECM-FICOLL 10 mg 商城订购
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