详细描述:
Matrix metalloproteinases (MMPs) are a family of endoproteases that require zinc and calcium for expressing catalytic activity. These enzymes play a central role in the maintenance and remodeling of the extracellular matrix. Elevated expression of their activity, caused either by upregulation of their expression or down-regulation of their cognate inhibitors, has been implicated in various degenerative disorders, including arthritis, cardiovascular disease, skeletal growth-plate disorders, and cancer metastasis. MMP-2 is a secreted collagenase with specificity toward Type IV, V, VII, and X collagens. Recombinant human MMP-2 produced in E. coli is a 64.7 kDa non-glycosylated, single polypeptide chain consisting of 576 amino acids (aa 110-660), fused to a 25 amino acid His-tag at the N-terminus and purified using proprietary chromatographic techniques.