详细描述：
The IL-1RL1 gene is a member of the IL-1 receptor family, encoding a transmembrane protein with a structure similar to IL-1R1. IL-1RL1 is a receptor for Interleukin-33; its stimulation recruits MYD-88, IRAK-1, IRAK-4, and TRAF-6, followed by phosphorylation of MAPK3/ERK1 and/or MAPK1/ERK2, MAPK14, and MAPK8. IL-1RL1 may possibly be involved in helper T-cell function. IL-1RL1 is highly expressed in kidney, lung, placenta, stomach, skeletal muscle, colon and small intestine. A soluble form of the IL-1RL1 is produced from the same gene by alternative splicing and was shown to be expressed in several cell types including fibroblasts and mast cells. Soluble IL-1RL1 also acts as a negative regulator of Th2 cytokine production and high levels have been reported in several disease states and conditions including asthma, sepsis and myocardial infarction. Analysis of the similar gene in mouse suggested that the IL-1RL1 receptor can be induced by proinflammatory stimuli, and may be involved in the function of helper T cells. IL-1RL1 produced in Sf9 cells is a single, glycosylated polypeptide chain (19-328 a.a.) fused to an 8 aa His-tag at the C-terminus. It contains a total of 318 amino acids and has a molecular mass of 36.0 kDa. IL -1RL1 shows multiple bands between 40-57 kDa on SDS-PAGE under reducing conditions and has been purified using proprietary chromatographic techniques.
