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氨基Tris-NTA， 1 mg/ml
Tris-NTA Amine, 1 mg/ml, 1 mg
His-tags are one of the most commonly used tags for protein expression analysis. Conventional metal ion chelators, such as nitrilotriacetic acid (NTA) and iminodiacetic acid (IDA), bind His-tags with low affinities in the range of 10 µM. The biotechrabbit Tris-NTA complexes three NTA groups that together bind a 6×His-tag with an affinity that is four orders of magnitude higher (1 nM) than is possible with conventional chelators (10 µM). The binding of His-tags is stoichiometric and single-molecule detection is possible. Binding is reversible: bound His-tags can be released with imidazole or ethylenediaminetetraacetic acid (EDTA). biotechrabbit™ Tris-NTA is available with a free amine group or conjugated to biotin. It can be used in a large range of applications, including protein detection and labeling, coupling proteins, lipids or cells to surfaces, protein purification and reversible protein modification.
特点：1、A complex of three Ni-NTA groups ensures high-affinity binding of His-tags；2、Binding affinity is approximately four orders of magnitude higher than monovalent metal ion chelators；3、Protein binding is stoichiometric 应用：Reversible labeling of proteins or cell surfaces；Detection and analysis of target molecules；Immobilization of proteins, lipids and cells on surfaces；Purification and sample preparation of proteins；Coupling with microscopic or spectroscopic probes 储存条件：4°C
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